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Figure 2S. Defect of myocardial cell differentiation and heart development in embryos injected with HIF-1α MO. Whole mount in situ hybridization analysis was carried out at stage 42 to detect cardiac troponin I (TnIc) mRNA expression in embryos injected with 40 ng of CMO (a) or HIF-1α MO1 (b,c) at 8-cell stage. TnIc mRNAs were visualized (blue) on transverse sections of the embryos injected with CMO (d) or HIF-1α MO1 (e,f). TnIc was asymmetrically detected in the embryos injected with HIF-1α MO1 compared to than those injected with CMO. This asymmetrical expression of TnIc reflects the interference of heart development (smaller heart in IF-1α MO1-injected embryos than CMO-injected embryos). nc, notochord; en, endoderm; h, heart Image published in: Nagao K et al. (2008) Copyright © 2008. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Larger Image Printer Friendly View

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