Xenbase Image ID: 138072

Fig. 6. Activities of ERK1/2 and AKT in limb stumps. Immunostaining was performed with anti-diphospho-ERK1/2 (A–C) and anti-phospho AKT substrate (D–E) at 1 day (A, D), 2 days (B, E) and 4 days (C, F). All sections are longitudinal sections. (A, B) At 2 days after amputation, activity of ERK1/2 was detected in bundles of axons in the stump (arrowheads). (C) At 4 days, however, diphosphorylation of ERK1/2 was evident in mesenchymal cells under the WE as well as regenerating axons. (D) Activity of AKT was extensively detectable in the mesodermal region of the stump at 1 day. Activity level of AKT in the distal region was low (bracket). (E) Activity of AKT was more evident at 2 days. (F) At 4 days, activated AKT-positive cells became restricted to the distal region where the majority of mesodermal cells are GFP positive. Scale bars: 200 μm. Image published in: Suzuki M et al. (2007) Copyright © 2007. Image reproduced with permission of the Publisher, Elsevier B. V. Gene Synonyms Species Stage(s) Tissue mapk1.S erk, erk1, erk2, ert1, MAP kinase, mapk, mapk1-a, mapk1-b, mapk2, mpk1, p42mapk, prkm1, prkm2, xp42 X. laevis Sometime during NF stage 56 to NF stage 58 regenerating hindlimb neuron proximal mesenchyme akt1.S akt-1, LOC108698803, pkb, protein kinase B, rac, v-akt, v-akt1, xAct X. laevis Throughout NF stage 54 regenerating hindlimb mesoderm proximal mesenchyme akt1.S akt-1, LOC108698803, pkb, protein kinase B, rac, v-akt, v-akt1, xAct X. laevis Sometime during NF stage 56 to NF stage 58 mesenchyme regenerating hindlimb distal mesenchyme mesoderm Image source: Published Larger Image Printer Friendly View

Return to previous page