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XB-IMG-138118

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Figure S2, Related to Figure 2. RNA-seq Read Profiles from replicates showing tra2b locus and confirmation of splice changes. (A) Top shows X. tropicalis tra2b gene transcript models with splice junction targeted by tra2b-MO2 boxed in blue. Shown beneath is read profiles from three biological replicates of control (black) and tra2b morphants (red). Blue box in read profiles indicates intron retention in tra2b morphants induced by tra2b-MO2. Arrows indicate variable exon 2, which shows increased inclusion in tra2b morphants. (B) RT-PCR confirmation of ESI events. (C) RT-PCR confirmation of RI events. Data shown are from X. tropicalis.

Image published in: Dichmann DS et al. (2015)

Copyright © 2015. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License.

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