XB-IMG-138120
Xenbase Image ID: 138120
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Figure S4, Related to Figure 4. Induced cardiac gene expression in animal caps
and wnt11b gene duplication in X. tropicalis. (A) Animal caps injected with
combinations of activin and wnt11b isoforms assayed for gata6b and sox17 by RTqPCR.
Coinjection of activin with wnt11b induces the cardiac mesoderm marker gata6b,
whereas coinjection of activin with wnt11-dn or wnt11-short does not. The endodermal
marker sox17 is induced by activin but not by either wnt11b isoform, as expected. Bar
plots show mean of three independent experiments + SEM of normalized fold-induction
compared to activin injected embryos. (B) JGI gene models for the annotated wnt11b
(right) and the adjacent duplication transcribed from the opposite strand (xetro.H00536;
left). RNA-seq read profiles show increased retention of intron4 in both genes in tra2b
morphants (blue dotted box). Image published in: Dichmann DS et al. (2015) Copyright © 2015. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Larger Image Printer Friendly View |