Xenbase Image ID: 138123

Figure 3 cnrip1 knockdown reduces expression of sox2, otx2, pax6 and rax. (A) qRT-PCR analysis for the pan-neural marker sox2 (stage 10.5 and 12.5), the anterior marker otx2 (stage 10.5 and 12.5), the eye and neural marker pax6 (stage 12.5 and 24) and the eye marker rax (stage 12.5 and 24) in embryos injected with control MO or cnrip1 MO (10 ng each/blastomere) into the animal region of two dorsal blastomeres at the 4-cell stage. The expression level of each gene was normalized to that of odc. Values are mean SD of three independent experiments; **P < 0.01; *P < 0.05. (B) Whole-mount in situ hybridization was carried out on MO-injected embryos. Control MO or cnrip1 MO (10 ng each) was injected into the animal region of the left dorsal blastomere at the 4-cell stage. The expression of sox2, otx2, pax6 and rax was reduced in 100% of cnrip1 MO-injected embryos (sox2, stage 10.5, n = 20; sox2, stage 12.5, n = 24; otx2, stage 10.5, n = 20; otx2, stage 12.5, n = 24; pax6, stage 12.5, n = 30; pax6, stage 24, n = 24; rax, stage 12.5, n = 24; rax, stage 24, n = 24). Anterior views with dorsal upwards. The representative results from two (sox2, stage 10.5; otx2, stage 10.5) or three (sox2, stage 12.5; otx2 stage 12.5; pax6, stage 12.5 and 24; rax, stage 12.5 and 24) independent experiments are shown. The black brackets indicate MO-injected left sides. The dashed lines indicate midlines of embryos. Image published in: Zheng X et al. (2015) Copyright © 2015. Image reproduced with permission of the Publisher, John Wiley & Sons. Gene Synonyms Species Stage(s) Tissue sox2.L anop3, LOC108718087, mcops3, Sox-2, XLSOX-2, Xsox-2, XSox2 X. laevis Throughout NF stage 10.5 anterior dorsal otx2.S otx-2, otx2-a, otx2-b, otxA, Xotx-2, Xotx2 X. laevis Throughout NF stage 10.5 anterior dorsal pax6.L an2, mgda, pax-6, pax6-a, pax6-b, wagr, XLPAX6, xpax6 X. laevis Throughout NF stage 12.5 neural plate anterior placodal area dorsal optic field rax.S rax-a, rax-b, rax1, rx, rx-1, rx1, rx1a, Rx2A, Xrax, Xrx1, Xrx1A X. laevis Throughout NF stage 12.5 anterior anterior placodal area optic field Image source: Published Experiment + Assay Source Phenotypes and Disease Xla Wt + cnrip1 MO + NF10.5 (in situ hybridization) fig.3.b Expression Phenotype decreased amount otx2.S expression in marginal zone decreased amount sox2.L expression in marginal zone Xla Wt + cnrip1 MO + NF12.5 (in situ hybridization) fig.3.b Expression Phenotype decreased amount otx2.S expression in pre-chordal neural plate decreased amount pax6.L expression in anterior placodal area decreased amount pax6.L expression in neural plate decreased amount pax6.L expression in optic field decreased amount rax.L expression in anterior placodal area decreased amount rax.L expression in optic field decreased amount sox2.L expression in neural plate Xla Wt + cnrip1 MO + NF24 (in situ hybridization) fig.3.b Expression Phenotype decreased amount pax6.L expression in brain decreased amount pax6.L expression in eye decreased amount rax.L expression in eye Larger Image Printer Friendly View

Return to previous page