Xenbase Image ID: 138128

Figure 8 Effects of cnr1 knockdown on expression of sox2, otx2, pax6 and rax. (A) qRT-PCR analysis for marker gene expression in embryos injected with control MO or cnr1 MO1 (20 ng each/blastomere) into the animal region of two dorsal blastomeres at the 4-cell stage. The expression level of each gene at stage 12.5 or 24 was normalized to that of odc. Values are mean SD of three independent experiments; *P < 0.05. (B, C) Whole-mount in situ hybridization analysis was carried out on embryos injected with 20 ng of control MO or cnr1 MO1 into the animal region of the left dorsal blastomere at the 4-cell stage. The representative results from two independent experiments are shown. Anterior views with dorsal upwards. The black brackets indicate MOinjected left sides. The dashed lines indicate midlines of embryos. (B) A mild reduction of pax6 expression in the cnr1 MO1- injected side was observed at stage 12.5 (52%, n = 21) and stage 24 (39%, n = 18). (C) A mild reduction of rax expression in the cnr1 MO1-injected side was observed at stage 12.5 (44%, n = 18) and stage 24 (56%, n = 16). Image published in: Zheng X et al. (2015) Copyright © 2015. Image reproduced with permission of the Publisher, John Wiley & Sons. Gene Synonyms Species Stage(s) Tissue pax6.L an2, mgda, pax-6, pax6-a, pax6-b, wagr, XLPAX6, xpax6 X. laevis Throughout NF stage 12.5 neural plate dorsal anterior placodal area optic field pax6.L an2, mgda, pax-6, pax6-a, pax6-b, wagr, XLPAX6, xpax6 X. laevis Throughout NF stage 24 optic vesicle diencephalon spinal cord rax.S rax-a, rax-b, rax1, rx, rx-1, rx1, rx1a, Rx2A, Xrax, Xrx1, Xrx1A X. laevis Throughout NF stage 12.5 optic field anterior placodal area neural plate rax.S rax-a, rax-b, rax1, rx, rx-1, rx1, rx1a, Rx2A, Xrax, Xrx1, Xrx1A X. laevis Throughout NF stage 24 optic vesicle stomodeal-hypophyseal primordium Image source: Published Experiment + Assay Source Phenotypes and Disease Xla Wt + cnr1 MO + NF12.5 (in situ hybridization) fig.8.b Expression Phenotype decreased amount pax6.L expression in anterior placodal area decreased amount pax6.L expression in neural plate Xla Wt + cnr1 MO + NF24 (in situ hybridization) fig.8.b Expression Phenotype decreased amount pax6.L expression in brain decreased amount pax6.L expression in eye Xla Wt + cnr1 MO + NF12.5 (in situ hybridization) fig.8.c Expression Phenotype decreased amount rax.L expression in anterior placodal area decreased amount rax.L expression in optic field Xla Wt + cnr1 MO + NF24 (in situ hybridization) fig.8.c Expression Phenotype decreased amount rax.L expression in eye Larger Image Printer Friendly View

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