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Fig. 2. Time course of mutations induced by microinjection with either Tyr-TALEN or Tyr-TALEN-DS mRNAs. Oocytes (squares) or fertilized eggs (circles) were injected with either Tyr-TALEN (open squares and circles) or Tyr-TALEN-DS (closed squares and a circle) mRNAs. The injected oocytes were treated with progesterone, transferred into a host female, and fertilized. The genomic DNA was extracted from four to six individual embryos at the indicated times. The target DNA was amplified by PCR and cloned. The DNA sequence was determined for five to fifteen clones per embryo (supplementary material Figs S1, S2). The data are expressed as the mean value ± standard error. The abscissa is indicated by hours (A) and days (B). Image published in: Nakajima K and Yaoita Y (2015) © 2015. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution license Larger Image Printer Friendly View

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