Xenbase Image ID: 138161

Fig. 5. Preferential mutagenesis in the testes of the m2 frog. (A) Simple direct sequencing of the PCR-amplified targeted genome region (DSP assay) using genomic DNA obtained from several organs and tissues of the Tyr-TALEN-DS-mRNA-injected m2 frog. The spacer sequence located between the Tyr-TALEN binding sites is shown at the top of the panel. The ambiguous sequence in the right and left testes begins with “A” (a red wave) and extends to the right, as shown by the red arrows, suggesting that these amplicons are mixtures of heterogeneous fragments with different mutations. (B) Mutated target sequences in organs and tissues of the m2 frog. The target DNA fragment was amplified using genomic DNA that was purified from organs and tissues and subcloned. The sequences of eight to 12 clones per organ or tissue were determined. The ratio of the number of the indicated sequence to the total number of sequences in an organ or tissue is shown in parentheses on the right. The alignment is labeled as described in the legend of Fig. 3F. (C) Mutation hit rate in several organs and tissues of m2. The mutation hit rate in an organ or tissue is indicated by a black bar, whereas the estimated mutation rate in the m2 spermatozoa is indicated by a white bar. Image published in: Nakajima K and Yaoita Y (2015) © 2015. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution license Larger Image Printer Friendly View

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