XB-IMG-138163
Xenbase Image ID: 138163
|
|
Fig. 2. Comparison of Xenopus laevis and human mapt genes. (A) To identify the exons, the nucleotide and the amino acid sequences of the four tau PCR
products from Fig. 1, as well as those of two previously characterized X. laevis mapt cDNAs (XTP-1 and XTP-2) cloned from late-stage tadpole tail (Olesen
et al., 2002), were aligned against nucleotides of the X. tropicalis genomic sequence and against the amino acid sequences of nine human tau isoforms (see
Supporting Information), respectively. The six Xenopus isoforms were generated by alternative splicing of four exons (exons 4, 4a, 6 and 12a) as indicated.
Exons that were constitutively present in all four isoforms are indicated in black. As a result of the insertion of exon 12a (124 nt), which has no human parallel,
into XTP-2, a frameshift is introduced and a premature stop codon (*) is encountered after the first amino acid of exon 13. (B) The degree of amino acid identity
[Identities (%)] as well as the number of amino acids (aa#) encoded for each exon of X. laevis and human tau. Image published in: Liu Y et al. (2015) Copyright © 2015. Image reproduced with permission of the Publisher, John Wiley & Sons. Larger Image Printer Friendly View |