Xenbase Image ID: 138164

Fig. 3. Tau protein expression began at early stages of axon outgrowth and increased with neuronal development through adulthood. (A–C) Parasagittal views of st. 22, 29/30 and 37/38 embryos processed for tau immunofluorescence in whole-mount and imaged by confocal laser-scanning microscopy. Images were taken with the 109 (NA 0.5) objective and represent maximal intensity projections created from stacks of 26, 17 and 20 optical sections, respectively, with voxel sizes of 1.8 9 1.8 9 8.0 lm (A and B) and 2.5 9 2.5 9 7.8 lm (C). At st. 22 (A), representing early axonal outgrowth, tau immunoreactivity was most intense in axon tracts within the hindbrain (arrow) and ventral spinal cord (S.C.), and was also visible in peripheral motor axons projecting to anterior somites. At st. 29/30 (B), representing a stage of maximal axonal outgrowth, tau immunoreactivity became more intense throughout the brain and spinal cord, and could be visualized in axons of the trigeminal nerve (Vmd, mandibular branch of the trigeminal nerve). By st. 37/38 (hatchling tadpole; C), tau immunoreactivity was abundant in axon tracts throughout the brain and spinal cord, in the retina (eye), and cranial nerves (X, vagus; IX, glossopharyngeal; VII, facial; Vmd). The image in (C) was cropped when rotated. (D) Tau immunoreactivity in neuronal perikarya and axons within a transverse section through the ventral diencephalon of a juvenile frog. The bracket indicates the optic tract. The image is a single optical confocal laser-scanning microscopy section taken with the 209 objective (NA 0.75), with a pixel size of 0.45 9 0.45 lm. (E and F) Tau immunoreactivity within transverse sections through the retina of a juvenile frog at low (E) and high (F) magnifications. Images are single confocal laser-scanning microscopy optical sections taken with the 209 objective (NA 0.75) with the scan zoom at 0.7 and 2.0, and pixel sizes of 0.60 9 0.60 lm and 0.45 9 0.45 lm for (E) and (F), respectively. Abbreviations: GCL, ganglion cell layer; INL, inner nuclear layer; IPL, inner plexiform layer; ONL, outer nuclear layer; OPL, outer plexiform layer; PL, photoreceptor layer. Scale bars: 100 lm (A–E); 20 lm (F). Image published in: Liu Y et al. (2015) Copyright © 2015. Image reproduced with permission of the Publisher, John Wiley & Sons. Gene Synonyms Species Stage(s) Tissue mapt.L tau, tau-like protein, xtp X. laevis Sometime during NF stage 22 to NF stage 37 and 38 nerve hindbrain trigeminal nerve optic tract glossopharyngeal nerve cranial nerve vagus nerve facial nerve neuron retinal outer plexiform layer axon motor axon retina optic nerve central nervous system brain spinal cord Image source: Published Larger Image Printer Friendly View

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