Xenbase Image ID: 138175

Fig. 4. Organ culture of Mprx1-GFP froglet forelimbs and effects of treatment with U0126 and LY294002. (A) Schematic representation of organ culture system. See text for details. (B) GFP expression in a control explant cultured for 2 days with DMSO, suggesting that the culture condition recapitulates the process of dedifferentiation. Treatment with U0126 at 20 μM (C) and 100 μM (D) and treatment with LY294002 at 20 μM (F) and 100 μM (G) inhibited up-regulation of GFP. Residual expression of GFP in the periosteum shows that these explants were derived from transgenic animals. (E, H) Additional incubation for 2 days with DMSO after treatment for 2 days with U0126 or LY294002 at higher concentrations induced up-regulation of GFP. (I) Numbers of BrdU- and phospho-Histone H3-positive cells in the explants. Treatments with U0126 and LY294002 repressed cell cycle progression. Asterisks represent 4 days of incubation (additional incubation for 2 days with DMSO). In all cases, bars represent the average of at least four explants. Scale bars: 200 μm. Image published in: Suzuki M et al. (2007) Copyright © 2007. Image reproduced with permission of the Publisher, Elsevier B. V. Larger Image Printer Friendly View

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