Xenbase Image ID: 138204

Fig. S4 Ionomycin can partially rescue pax8 expression in Pkd2-MO morphant embryos. MO-Pkd2 was injected into the two left blastomeres at the 4-cell stage, and embryos cultured until late gastrula stage (NF st12.5) at which time ionomycin- or control DMSO-soaked beads were implanted into dorso-lateral mesoderm. Beads stayed in contact with dorso-lateral mesoderm until neurula stage (NF st14-15) after which they were generally expelled out of the embryo or fell into the archenteron. Embryos were further cultured until late gastrula stage for ISH analysis of pax8 expression. Three kind of patterns can be observed on injected sides, grading from very weak (Gr1), intermediate (Gr2) and strong (Gr3) pax8 expression. Scoring of these patterns reveals that the proportion of Gr3 patterns is significantly increased in ionomycin-bead implanted embryos (Pearson’s chi-square test of independence p<0,05). Arrows indicate orientation of AP axis. Image published in: Futel M et al. (2015) Copyright © 2015. Image reproduced with permission of the Publisher, The Company of Biologists Ltd. Larger Image Printer Friendly View

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