Xenbase Image ID: 138210

Figure 1. XREST/NRSF is required for morphogenesis and neuronal gene expression during early stages of neurogenesis. A, Anterodorsal views and transversal sections of embryos fixed at late neurula, injected with 500 pg of dnXREST or 5 pmol of MoXREST (indicated on top) in one cell at the 2-cell stage; the injected side in all figures is to the left. Embryos exposed to dexamethasone at late blastula [Dex(+) st. 9] and embryos injected with MoXREST exhibit an apparent loss of expression of neuronal genes in neural tube (arrows) and in the trigeminal placode (arrowheads). Late gastrula induction [Dex(+) st. 12] results in less marked effects. Transversal sections show altered morphology of neural tube and confirm the diminished expression of neuronal genes (arrows). Probes used for ISH are indicated to the left of each row. B, Lateral views of stage 37 embryo injected in one cell at the 2-cell stage with 500 pg of dnXREST and induced at late blastula stage. Note the defect of eye development in the injected side (bottom, arrow). Image published in: Olguín P et al. (2006) Copyright © 2006. This image is reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Gene Synonyms Species Stage(s) Tissue scn2a na(v)1.2, nav1.2 X. tropicalis Sometime during NF stage 22 to NF stage 37 and 38 neural tube trigeminal placode stmn2 scg10, scgn10, sgc10, stmn2-a, stmn2-b X. tropicalis Sometime during NF stage 22 to NF stage 37 and 38 neural tube trigeminal placode tubb2b N-Tub, n-tubulin, NBT, neural beta-tubulin, NST, ntubulin, Xn-tubulin X. tropicalis Sometime during NF stage 22 to NF stage 37 and 38 neural tube trigeminal placode Image source: Published Larger Image Printer Friendly View

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