Xenbase Image ID: 138214

Figure 5. XREST loss of function reverts the effect of Bmp4 misexpression on ectodermal patterning. Anterior views of stage 18 embryos injected with 500 pg of dnXREST, alone or in combination with 500 pg of Bmp4 mRNA (indicated on top). Probes used for ISH are indicated to the left of each row, and the stage of dexamethasone addition is indicated on top. The injected side of not induced dnXREST/BMP4 embryos exhibits the loss or decrease of neural ectoderm revealed by the domain of Sox2 expression (c, arrows, compare with a), the ectopic expression of keratin (g, arrow, compare with e), and the dorsal displacement (brackets) and contraction (arrowhead) of Slug expression domain (k, compare with i) (brackets). The activation of dnXREST at late blastula stage results in the restoration of anterior neural plate size of BMP4 misexpressing embryos. Note the recovered expression of Sox2 (d), keratin (h), and Slug (l) in relation to the neural plate size (brackets). Nevertheless, loss of keratin (f) and Slug (j) expression observed in dnXREST-injected embryos induced at stage 9 was not efficiently rescued by the BMP4 coexpression (h, l, arrows). Image published in: Olguín P et al. (2006) Copyright © 2006. This image is reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Gene Synonyms Species Stage(s) Tissue sox2.S anop3, LOC108718087, mcops3, Sox-2, XLSOX-2, Xsox-2, XSox2 X. laevis Throughout NF stage 18 neural plate krt12.4.L ck81, cytok, cytokeratin, E-keratin, epi-keratin, epidermal keratin, Epidermis-specific keratin, EpiK, epiker, EpK, epK, K81, xK81, xk81a, xk81a1 X. laevis Throughout NF stage 18 epidermis non-neural ectoderm snai2.L slug, snai2-a, snai2-b, Snail2, xSlu, xslug, XSnail2 X. laevis Throughout NF stage 18 neural crest neural plate border Image source: Published Larger Image Printer Friendly View

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