XB-IMG-138268
Xenbase Image ID: 138268
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Fig. 5. Northern blot of RNA from dissected parts of late
(stl8) neurulae, probed successively for transcripts from
the XMyf5, cardiac actin (Mohun et al. 1984), and EF-lo-
(Krieg et al. 1989) genes. Cardiac actin is a striated muscle
marker, and EF-la- shows the relative amounts of total
RNA in each lane. Parts were pooled from five dissected
embryos; RNA from one whole embryo was analysed for
comparison. The dorsal endoderm and notochord lanes
were relatively underloaded in this experiment. Two other
experiments, in which relatively more endodermal RNA
was used, showed that the endoderm does not contain a
significant concentration of XMyf5 RNA at stl3 or at stl8
(data not shown). That the notochord does not express
XMyf5 was shown by in situ hybridization (Fig. 4A,C). Dl,
dorsal; vl, ventral; end, endoderm; mes, mesoderm; ect,
ectoderm. Image published in: Hopwood ND et al. (1991) Copyright © 1991. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Larger Image Printer Friendly View |