XB-IMG-138273
Xenbase Image ID: 138273
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Fig. 2. (A) Triton extracts from stage 5, 17 and 38
embryos Western blotted with Z9 serum. Three bands in
the A/r range 55-57000 are visible at each stage (arrowed).
The level of the 57k band declines relative to the two
lower Mr bands as development procedes. The cross
reacting proteins at a lower Mr at stage 38 are degradation
products of the major bands odserved. 1 and p are the two
yolk proteins lipovitellin and phosvitin. (B) Western blots
of Triton extracts of uninjected oocytes, oocytes injected
with viml mRNA and oocytes injected with vim4 mRNA.
The antibody used was affinity-purified Z9, which does not
react with yolk proteins. (C) Triton extract of 15 oocytes
blotted with affinity-purified Z9, showing again that there
is no cross reaction of the affinity-purified antibody with
yolk proteins. Image published in: Torpey NP et al. (1990) Copyright © 1990. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Larger Image Printer Friendly View |