Xenbase Image ID: 138316

Fig. 2. Manually separating CNC from the surrounding tissue partially rescues CNC migration induced by ADAM13 knock down. (A) Schematics of the experimental procedure. Either GFP mRNA alone, or a mixture of GFP mRNA and ADAM13 morpholino was injected into the dorso-animal cell at the 8-cell stage. At stage 15, embryos were sorted for their expression of GFP and underwent one of the following procedures. 1—The ectoderm was peeled off (ecto peel). 2—The ectoderm was peeled off and the crest lifted away from the underlying mesoderm (ecto+meso peel). 3—The CNC were excised from the embryo and placed back in (autograft). 4—After the procedure, the ectoderm was pulled back on and left to heal. The embryo was left to develop until tailbud stage and scored for their lack of CNC migration. (B) The histogram represents the percentage of embryos with no CNC migration normalized to embryos injected with GFP alone and corresponds to the mean of three independent experiments. As previously reported, ADAM13 KD significantly inhibits CNC migration in the targeted injection assay. The ectoderm peel procedure did not affect this inhibition. However, the ectoderm+mesoderm peel and the autograft procedures rescued CNC migration to levels not statistically different from the GFP control. The number of embryos scored was as follows: GFP; n=159, MO13; n=154, MO13 ecto peel; n=47; MO13; n=45; MO13 autograft; n=43. The error bar represents standard deviation from the mean. ⁎=Statistically different from GFP (p<0.05). Image published in: Cousin H et al. (2012) Copyright © 2012. Image reproduced with permission of the Publisher, Elsevier B. V. Larger Image Printer Friendly View

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