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Fig. 6. The partial knock down of Cadherin-11 does not rescue CNC migration of ADAM13 morphants. Embryos were injected at the 1 cell stage with either ADAM13 morpholino (MO13 1 ng) alone or together with various doses of morpholino against Cadherin-11 (MOC11) ranging from 5 ng to 25 ng. At stage 20, the proteins of 10 embryos equivalent were extracted, immunoprecipitated with either the cadherin-11 mAb 1B4 or ADAM13 rabbit pAb 6615F and blotted with the cadherin rabbit pAb C11 and the ADAM13 goat pAb 877 respectively. The knock down of ADAM13 alone leads to an increase of the full-length form of Cadherin-11 that can be decreased by a partial cadherin-11 knock down using 5 ng of MOC11. (B) Embryos were injected at the 8-cell stage with either ADAM13 morpholino (MO13 1 ng) alone or together with various doses of morpholino against Cadherin-11 (MOC11) ranging from 1 ng to 5 ng. The mRNA encoding GFP was either co-injected or injected alone as lineage tracer. Embryos were raised until stage 28 and scored for their lack of CNC migration. The histogram represents the percentage of embryos displaying no CNC migration normalized to embryos injected with RFP alone and corresponds to the mean of four independent experiments. The partial knock down of Cadherin-11 with either 1 or 2.5 ng of MOC11 did not significantly rescue the inhibition of CNC migration induced by ADAM13 knock down. The co-injection of 5 ng of MOC11 produced a significantly more severe inhibition than ADAM13 knock down alone. The error bars represent standard deviation from the mean. The number of embryos scored for each case ranged between 207 and 218. *=Statistically different from MO13 (p<0.05). Image published in: Cousin H et al. (2012) Copyright © 2012. Image reproduced with permission of the Publisher, Elsevier B. V. Larger Image Printer Friendly View

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