Xenbase Image ID: 138365

Figure 2 Colocalization of Csp with CFTR or with calnexin in Calu-3 cells. Calu-3 cells were labeled with polyclonal Csp antiserum and monoclonal CFTR R-domain or calnexin antibodies. Labeling was detected by Alexa488 (greenindicating Csp) or Alexa568 (red indicating CFTR inpanels A–E or calnexin in panel F). Serial images in the xy plane were collected every 0.5 μm through the specimen depth; xy images (panels A–D), taken from cell apex to base, are separated by 2.5 μm. Panel E shows the xz scan of the region indicated in panel A. Scale bar, 10 μm. Images were collected by confocal microscopy and analyzed using Metamorph (Universal Imaging) software. Image published in: Zhang H et al. (2002) Copyright © 2002. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Larger Image Printer Friendly View

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