Xenbase Image ID: 138371

Figure 8 Csp coexpression reduces oocyte CFTR protein levels. cRNAs encoding CFTR (1 ng) and Csp isoforms (5 ng) were injected into Xenopus oocytes as indicated. Upper panel, results from CFTR immunoprecipitation. Immunoprecipitation was performed with anti-CFTR monoclonal M3A7, and the immunoblot was probed with anti-CFTR polyclonal R3195 antibodies. Lane 1, uninjected control; lane 2, CFTR alone; lane 3, CFTR plus Csp1; lane 4, CFTR plus Csp2; lane 5, CFTR plus Csp2 double mutant; lane 6, HEK293 cells transiently expressing CFTR used as a positive control. Lower panel, cAMP-stimulated chloride currents recorded from the oocyte populations used for the immunoprecipitations. Data are from five pooled oocytes for immunoprecipitation (50 μg of protein/lane) and four or five oocytes for ΔI Cl under each condition. Recordings/immunoprecipitations were performed 4 days after injection. Image published in: Zhang H et al. (2002) Copyright © 2002. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Larger Image Printer Friendly View

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