Xenbase Image ID: 138373

Figure 10 Pulse-chase analysis of CFTR biogenesis in HEK293 cells, performed as described under “Experimental Procedures.” TTF indicates the transient transfection conditions. 35S-Labeled CFTR was immunoprecipitated using 3 μg of monoclonal CFTR antibody M3A7. Samples were resolved on SDS-PAGE, and CFTR was revealed by autoradiography. The intensities of CFTR C and B forms at the indicated times were quantified by densitometry and are expressed as a percentage of CFTR B form at chase time = 0 (taken as 100%). Image published in: Zhang H et al. (2002) Copyright © 2002. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Larger Image Printer Friendly View

Return to previous page