Xenbase Image ID: 138453

Figure 3. Comparison of Nocturnin and PARN Protein Expression in the Retina Western blot analysis was performed on Xenopus laevis retinal proteins collected at 4-hr intervals throughout the 24-hr day/night cycle. (A) Using an affinity-purified antibody generated against nocturnin amino acids 51–69, we show that nocturnin is expressed with circadian rhythmicity in the retina, with peak levels at mid- to late night. (B) PARN protein is expressed constitutively in the retina, with equivalent expression levels throughout the 24-hr cycle. After nocturnin or PARN protein was examined, the blots were stripped and reprobed with an antibody that recognizes actin to control for equal loading (bottom panel). ZT refers to zeitgeber time; ZT 0 is the onset of light, and ZT 12 is lights-off in a 12 hr light/12 hr dark cycle. Image published in: Baggs JE and Green CB (2003) Copyright © 2003. Image reproduced with permission of the Publisher, Elsevier B. V. Larger Image Printer Friendly View

Return to previous page