Xenbase Image ID: 138480

S6 Fig. Statistical analysis of pre-rRNA levels by Ratio Analysis of Multiple Precursors (RAMP) [60]. RNAs were quantitated from northern blots using a phosphorimager (Bio-Rad Personal Molecular Imager). All ratios are representative of three biological replicates (n = 3). The levels of all RNAs were normalized to the uninjected control (UC). All statistical analyses for significance for Nol11-depleted embryos (NOL11) were performed compared to the control morpholino injected embryos (CMO). A. Pre-rRNA levels in Nol11-depleted embryos are not significantly affected at stage 12 compared to CMO as shown by a probe in the 5’ETS (probe a). B. At stage 28, the levels of 40S and 20S pre-rRNAs, are significantly decreased relative to the loading control 7SL RNA for Nol11-depleted embryos compared to CMO as shown by probe a. This is consistent with decreased pre-rRNA transcription. C. Pre-rRNA levels relative to the 7SL RNA for Nol11-depleted embryos are not significantly affected compared to CMO at stage 12 as shown by a probe in the ITS1 (probe c). D. At stage 28 for Nol11-depleted embryos, the 40S, 36S, and 20S pre-rRNAs are all significantly decreased relative to the 7SL RNA compared to CMO as shown by probe c. This is consistent with decreased pre-rRNA transcription. However, the levels of 19S are significantly increased relative to the 7SL RNA compared to CMO as this precursor accumulates in Nol11-depleted embryos. This is indicative of a pre-rRNA processing defect. E. and F. For both stage 12 and 28 Nol11-depleted embryos, the ratios of 19S/40S, 19S/36S, and 19S/20S are significantly increased compared to CMO indicating accumulation of the 19S pre-rRNA relative to the other three pre-rRNAs that hybridize with probe c. This is indicative of a pre-rRNA processing defect. [ns = p>0.05 (not significant), * = p≤0.05, ** = p≤0.01, *** = p≤0.001, **** = p≤0.0001] doi:10.1371/journal.pgen.1005018.s006 Image published in: Griffin JN et al. (2015) Image reproduced on Xenbase with permission of the publisher and the copyright holder. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution license Larger Image Printer Friendly View

Return to previous page