Xenbase Image ID: 138555

Figure 1. Notum Antagonizes Wnt Signaling in Mammalian Cells and Xenopus Embryos (A) Notum inhibited TOPFLASH induced by Wnt3a in HEK293T cells. Increasing doses of a Notum expression vector were co-transfected with an expression vector for Wnt3a. (B) Notum inhibited TOPFLASH induced by the recombinant WNT3A protein. HEK293T cells transfected with increasing doses of the Notum expression vector were incubated with the WNT3A protein. (C) Notum(S239A) lacked the ability to inhibit TOPFLASH induced by the WNT3A protein. (D) Notum inhibited axis duplication induced by Xenopus Wnt8, but not β-catenin, while Notum(S239A) did not inhibit axis duplication induced by either Wnt8 or β-catenin. n, embryos examined. (E–I) Notum in animal pole explants inhibited expression of Xnr3 induced by Wnt8 (E), but not by β-catenin (F), nor did it inhibit Xbra expression induced by Nodal/Xnr1 (G), or bFGF (H), or Vent2 expression induced by BMP4 (I). EF1α, a loading control; uninjected embryos, uninj.; whole embryos, WE; whole embryos without the reverse transcriptase, -RT. There were two doses of Notum mRNA that were injected. Error bars (A–C) represent SD of triplicated experiments. See also Figure S1. Image published in: Zhang X et al. (2015) Copyright © 2015. Image reproduced with permission of the Publisher, Elsevier B. V. Larger Image Printer Friendly View

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