Xenbase Image ID: 138565

Figure 5. Notum Is Required for Anterior Development in Xenopus Embryos (A and B) Dorsal injection of Notum mRNA, but not Notum(S239A), induced an enlarged head similar to that induced by Tiki1 mRNA, and resulting phenotypes were tabulated. (C) The Notum MO and the Notum’ MO inhibited protein synthesis from Xenopus Notum (xNotum) or Notum’ (xNotum’), but not mouse Notum (mNotum) mRNA. Co, MO, and MO’ indicate control MO and MOs against xNotum and xNotum’, respectively. (D and E) Dorso-animal injection of the two Notum MOs together caused anterior defects that were rescued by mNotum mRNA, and resulting phenotypes were tabulated. (F) The Notum MOs suppressed expression of forebrain markers Otx2 and Bf1 and a hindbrain marker Krox20 at stage 17. See also Table S1. (G) Illustration of the MO-injected A1 and B1 blastomeres at 32-cell stage and their descendent tissues at stage 10.5. (H) The Notum MOs injected into the A1 blastomeres caused anterior defects in more embryos (69%) than those injected into the B1 blastomeres (22%). See also Figures S4 and S5; Tables S2 and S3. Image published in: Zhang X et al. (2015) Copyright © 2015. Image reproduced with permission of the Publisher, Elsevier B. V. Larger Image Printer Friendly View

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