Xenbase Image ID: 138566

Figure 6. Notum Is Required for Neural Induction in Embryos and Animal Explants (A) The Notum MOs reduced the expression domain of Sox2, a pan-neural marker, and expanded the expression domain of cytokeratin, an epidermal marker, and the reciprocal changes were rescued by mNotum, but not mNutum(S239A), mRNA. See also Table S4. (B) The Notum MOs suppressed expression of neural markers induced by injection of the Chordin mRNA and restored cytokeratin expression that were inhibited by Chordin. The effect of Notum MOs was rescued by mNotum, but not mNotum(S239A), mRNA; a cement gland marker, XAG; anterior neural markers, Bf1 and Pax6; a pan-neural marker, Sox2; a neuronal marker, N-tubulin; an epidermal marker, Keratin; a mesodermal marker, M-Actin; and a loading control, ODC. (C) Chordin induced Bf1 and Sox2 expression when the Notum MOs and a β-catenin MO were co-injected. (D) Injection of Notum mRNA, like that of Chordin mRNA, induced expression of neural markers Sox2 and Pax6 and suppressed that of an epidermal marker, cytokeratin, in animal cap explants. See also Figure S6. Image published in: Zhang X et al. (2015) Copyright © 2015. Image reproduced with permission of the Publisher, Elsevier B. V. Larger Image Printer Friendly View

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