Xenbase Image ID: 138578

Figure S4. Characterization of Xenopus laevis Notum and Notum’. Related to Figure 5. This figure provides additional data demonstrating that Xenopus laevis Notum and Notum’ behave like mouse Notum and inhibit Wnt signaling through Wnt deacylation. Appears before Figure 5. (A) Xenopus laevis Notum (xNotum) and Notum’ (xNotum’), like mouse Notumm(mNotum), inhibited Wnt3a-induced TOPFLASH. Error bars represent SD of triplicated experiments. (B) xNotum inhibited Xnr3 induction by Wnt8 in animal pole explants. Two doses of xNotum mRNA (100 and 200pg) were used. (C) The Wnt3a protein modified by mouse Notum or xNotum or xNotum’ became hydrophilic in the Triton X-114 detergent-aqueous phase separation assay. (D) xNotum and xNotum’, like mNotum, diminished Wnt3a acylation when each was coexpressed with Wnt3a in HEK293T cells Image published in: Zhang X et al. (2015) Copyright © 2015. Image reproduced with permission of the Publisher, Elsevier B. V. Larger Image Printer Friendly View

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