XB-IMG-138580
Xenbase Image ID: 138580
Figure S6. Notum is required for neural induction by Noggin, but is not required for Noggin or Chordin to antagonize BMP signaling. Related to Figure 6. This figure provides additional data supporting that Notum is required for neural induction.
Appears after Figure 6.
(A) Neural marker induction by the Noggin protein was suppressed by the Notum MOs in the animal pole explants, and was rescued by co-injection of mNotum, but not Notum(S239A), mRNA. XAG, a cement gland marker; Bf1 and Pax6, anterior neural markers; Sox2, a pan-neural marker; N-tubulin, a neuronal marker; M-Actin, a mesodermal marker; ODC, a loading control.
(B) The Notum MOs did not affect Chordin or Noggin activity in antagonizing BMP4 induction of Vent2 expression in animal pole explants. Image published in: Zhang X et al. (2015) Copyright © 2015. Image reproduced with permission of the Publisher, Elsevier B. V. Larger Image Printer Friendly View |