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S3 Fig. Acute HDAC1 knockdown does not block visual deprivation induced increase of proliferative cells. (A). A cartoon showing that stage 46 tadpoles were placed in a 12h/12h dark/light incubator for 96 hrs (Ctrl), or put into a dark box for 48 hrs after 2 days of dark/light cycle (VD), or electroporated with HDAC1-MO and immediately placed in a dark box for 48 hrs after 2 days of dark/light cycle (acute HDAC1-MO+VD). Tadpoles were incubated with BrdU for immunostaining at stage 49. (B) Fluorescent images showing representative BrdU-labeled cells in control (left panel), VD (middle panel) and acute HDAC1-MO+VD (right panel) tadpoles. Scale: 50 μm. (C). Quantification data showed that visual deprivation increases the number of BrdU-labeled cells but acute HDAC1-MO transfection and VD does not change the total number of proliferative cells compared to VD-exposed tadpoles. N = 4, 6, 5, for Ctrl, VD and HDAC1-MO+VD, respectively, ***p<0.001. doi:10.1371/journal.pone.0120118.s003 Image published in: Tao Y et al. (2015) Image reproduced on Xenbase with permission of the publisher and the copyright holder. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution license Larger Image Printer Friendly View

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