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Fig. 3. N-CAM is expressed on the hindlimb muscles of tadpoles and on adult leg muscles after denervation. A shows a cross section of a hindlimb of a stage-55 tadpole in phase contrast, and B shows the immunofluorescence staining of the same section obtained with the monoclonal antibody 4D. Note the strong staining of the muscle fibres with N-CAM antibodies. C is the phase-contrast image of a hindlimb muscle of a comparably staged tadpole, and D is the staining pattern obtained with polyclonal anti-frog N-CAM antibodies. E-F display the N-CAM immunofluorescence staining of gastrocnemius muscles from the same adult frog with polyclonal anti-frog N-CAM antibodies; E is from the intact limb and F is the contralateral leg 4 days after denervation. The bright spots in E are autofluorescing red blood cells. Bar, 135um. Image published in: Kay BK et al. (1988) Copyright © 1988. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Larger Image Printer Friendly View

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