XB-IMG-138624
Xenbase Image ID: 138624
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Fig. 5. Lack of N-CAM expression
in cultured Xenopus myotomal
muscle cells. Tail muscle cells from
stage-22 embryos were cultured
and reacted in the living state with
N-CAM antibodies and FITCconjugated
secondary antibodies
(A), or examined by phasecontrast
microscopy (B). Cells
were also double stained with
N-CAM antibodies (C) and
R-BTX (D); the spontaneously
formed acetylcholine receptor
clusters (marked with arrowheads
in D) do not react with N-CAM
antibodies. Neurites and growth
cones (arrowhead) from neural
tube explants, on the other hand,
react quite well with N-CAM
antibodies (E). Muscle cells from
stage-50 tadpoles were also
examined for N-CAM expression
after dissociation from the tail
musculature. After dissociation,
these cells were immediately fixed
and processed for binding of
N-CAM antibodies (F) or R-BTX
(G). Note the lack of N-CAM at
the postsynaptic acetylcholine
receptor clusters, marked by
arrowheads in G, as well as along
the extrajunctional sarcolemma.
Bar, 30um. Image published in: Kay BK et al. (1988) Copyright © 1988. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Larger Image Printer Friendly View |