Xenbase Image ID: 138648

Fig. 4. XEZ expression is anteriorly restricted. Dissection series of stage 25 Xenopus laevis embryos analysed by RT-PCR. Stage 25 embryos were dissected as shown in the insert picture into three equivalent axial pieces, A, anterior; M, middle; P, posterior; the endoderm was removed. Duplicate samples 1 and 2 both consisted of 5 embryos. Analysis shows the expression of XEZ is highest in the anterior nervous system, is reduced in the middle section and is barely detectable in the posterior in both dissected embryo pools. Whole embryo control cDNA at stage 25 was used for the linearity and EF1α was used as a loading control. Since the sections of neural tissue were equal in terms of regional identity, but only approximately equivalent in terms of amount of biological material, it is not reasonable to equalize loading of cDNA between samples (as shown by the EF1α signal). Image published in: Barnett MW et al. (2001) Copyright © 2001. Image reproduced with permission of the Publisher, Elsevier B. V. Larger Image Printer Friendly View

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