Xenbase Image ID: 138649

Fig. 5. XEZ is induced by noggin. RT-PCR showing induction of XEZ in noggin-injected animal caps. Animal caps were taken from stage 9 embryos previously injected at the 1 cell stage with noggin mRNA. The animal caps were cultured to stage 13, 20, 28 and 32. RT-PCR analysis shows that at stage 13 XEZ expression is highly induced in noggin animal caps compared to a much lower level of expression in control animal caps. However, this high level of expression is not maintained and XEZ expression is only slightly higher in animal caps overexpressing noggin compared to control caps at stages 20, 28 and 32. Stage 32 whole embryo cDNA was used as input for the linearity and EF1α was used as a loading control. Image published in: Barnett MW et al. (2001) Copyright © 2001. Image reproduced with permission of the Publisher, Elsevier B. V. Larger Image Printer Friendly View

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