Xenbase Image ID: 138651

Fig. 7. Repression of En-2 by XEZ. Embryos were uni-laterally co-injected at the two cell stage with XEZ mRNA and FLDX. The FLDX was used as a marker for the injected side. The embryos were cultured to stage 19 and subjected to wholemount in situ hybridisation using an En-2 antisense RNA DIG-labelled probe. The injected side is indicated by an arrow. (A) Complete ablation of En-2 expression on XEZ injected side. (B) Anterior shift of En-2 expression on XEZ injected side. (C) Repression of En-2 expression on XEZ injected side. (D) Normal control. The En-2 sense control probe showed no staining pattern (data not shown). Image published in: Barnett MW et al. (2001) Copyright © 2001. Image reproduced with permission of the Publisher, Elsevier B. V. Larger Image Printer Friendly View

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