Xenbase Image ID: 138652

Fig. 8. XEZrepresses En-2. (A) Xenopus laevis embryos were uni-laterally co-injected at the 2-cell stage with XEZmRNA and FLDX. The embryos were cultured to stage 19 and subjected to wholemount in situ hybridisation using the midbrain-hindbrain junction marker En-2 antisense RNA probe. The embryos were scored as having reduced expression of En-2when the level of En-2 staining was lower (or absent) than control embryo normal variation. The embryos showing reduced-expression were also scored for any shift in the anterior or posterior register of the En-2 marker staining relative to the uninjected side. Numbers in brackets are actual numerical scores. (B) XEZ represses En-2, Krox-20 but not other neural markers. Embryos were injected at the 1 cell stage with XEZmRNA, cultured to stage 19 and subjected to RT-PCR. The figure shows that the expression of En-2 and Krox-20 are repressed compared to uninjected control, but other neural markers are not affected. EF1α was used as a loading control and the uninjected sample used for linearity input cDNA. Image published in: Barnett MW et al. (2001) Copyright © 2001. Image reproduced with permission of the Publisher, Elsevier B. V. Larger Image Printer Friendly View

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