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Fig. 4. Loss of Xfoxi1a/b function leads to reduction of epidermal tissue in naïve ectodermal cells. Animal caps given injection of Xfoxi1a-MO (50 ng), Xfoxi1b-MO (50 ng), Xfoxi1a-MO+Xfoxi1b-MO (25 ng each) or five-base mismatched control MOs for Xfoxi1a and Xfoxi1b (25 ng each) were excised from stage 9 embryos, and then cultured until sibling embryos reached stage 14. RT-PCR was performed using primers to detect the neural marker Sox2, non-neural ectodermal markers (XK81, Dlx3, Msx1) and the mesodermal marker MyoD. H4 (histone H4) was used as the loading control. Image published in: Matsuo-Takasaki M et al. (2005) Copyright © 2005. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Larger Image Printer Friendly View

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