Xenbase Image ID: 138809

Fig. S7. Validation of pqbp1 knockdown effects on fgf4 and cdx4 expression in X. laevis and X. tropicalis embryos. Results were analyzed and plotted as described in Fig. 5. A-C. Expression of fgf4 and cdx4 was analyzed by qPCR. A. Either of the PQBP1 MOs (at 50 ng) reduced the expression of fgf4 and cdx4 in X. laevis morphant embryos, *P < 0.05 or **P < 0.01, with comparisons to control embryos (CT). B. Reduced fgf4 and cdx4 expression in embryos injected with 30 ng pqbp1 MOb at the 4-cell stage into the VMZ was rescued by co-injection of MOb-resistant PQBP1 mRNA (0.4 ng or 2 ng) but not control b-gal mRNA (-); *P < 0.05 or **P < 0.01, with comparisons to MO-only injected embryos (-). C. Analysis of PQBP1 knockdown in X. tropicalis embryos. X. tropicalis PQBP1 MO (20 ng) was bilaterally injected into embryos at the 2-cell stage, and phenotypes of stage-matched wild type (WT) embryo (left panel) and MO-injected embryos (middle panel) are shown. Expression of fgf4 and cdx4 were evaluated in WT and pqbp1 morphant X. tropicalis at the gastrula stage (right panel), as described in Fig. 5; *P < 0.05 or **P<0.01, with comparisons to control embryos (CT). Image published in: Iwasaki Y and Thomsen GH (2014) Copyright © 2014. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Experiment + Assay Source Phenotypes and Disease Xtr Wt + pqbp1 MO + NF10.5 (RT-PCR) Fig.S7.C Expression Phenotype decreased amount cdx4 expression in the embryo decreased amount fgf4 expression in embryo Xtr Wt + pqbp1 MO + NF32 (whole-mount microscopy) Fig.S7.C Anatomical Phenotype absent tail bud decreased length of anterior-posterior axis decreased size of the head decreased size of the tail bud Disease Renpenning syndrome Larger Image Printer Friendly View

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