XB-IMG-138874
Xenbase Image ID: 138874
|
FIG. 9. Localization of XLPOU 1 transcripts in sagittal (A) and horizontal sections (B, C) of stage 25 embryos by in situ hybridization. In the
top panel, A is a schematic of an embryo sectioned in the sagittal plane. A’ and A” are micrographs of sections, visualized by dark-field optics,
that were hybridized to the sense probe (control) or antisense probe of XLPOU 1 OP, respectively. There are air bubbles present in the endoderm
in both the control and the experimental material; under the microscope, the silver grains can easily be distinguished from the air bubbles. The
middle panel (B) shows a stained (hemalum and eosin, H&E), bright-field image of an embryo sectioned in the horizontal plane. The corresponding
dark-field image (in the opposite orientation to the bright-field image) shows hybridization to the antisense probe of XLPOU 1 OP
(B’). The bottom panel (C) shows a stained (H&E), phase-contrast micrograph of an embryo (head region only) sectioned in the horizontal plane
at the level of the eye. The corresponding dark-field image (in the opposite orientation to phase-contrast image) shows hybridization to the
antisense probe of XLPOU 1 OP (C’). Sections were exposed for 8 weeks at 4’C. The brain (br), cement gland (cg), eye (e), somites (so), and nerve
cord (nc) are indicated. Image published in: Agarwal VR and Sato SM (1991) Copyright © 1991. Image reproduced with permission of the Publisher, Elsevier B. V.
Image source: Published Larger Image Printer Friendly View |