Xenbase Image ID: 139011

FIGURE 4. Hspa5 depletion inhibits of pronephric duct and tubule formation. Single vegetal-ventral blastomeres of eight-cell stage embryos were injected with LacZ mRNA (100 pg/embryo) and Hspa5MO2 (30 ng/embryo). Injected embryos were collected at approximately stage 39. Embryos were stained for LacZ and immunostained with 3G8 and 4A6 antibodies to detect pronephric ducts and tubules. A, A , B, and B , pronephric tubules in embryos detected by 3G8 antibody. C, C , D, and D , pronephros ducts in stage 39 embryos detected by 4A6 antibody. A –D , higher magnification of A–D. White arrow indicates pronephros. pn, pronephros. The white arrow indicates tubule in A–B and duct in C–D . Uninjected, uninjected side; MO-lacZ, Hspa5MO2-injected side. Image published in: Shi W et al. (2015) Copyright © 2015. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Larger Image Printer Friendly View

Return to previous page