XB-IMG-139012
Xenbase Image ID: 139012
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FIGURE 5. Pronephros induction in animal cap assays. A, schematic diagram of in vitro pronephros induction assay showing animal caps are induced to
differentiate into pronephros tissues using atRA and activin. B and C, Hspa5MO2 (MO2) was injected into both blastomeres at the two-cell stage. Animal caps
were dissected at stage 9 and treated with activin and atRA for 3 h. Subsequently, the animal caps were transferred into normal animal cap culture mediumand
cultured until stage 15 (St15) (B) or stage 32 (St32) (C). Expression analysis was performed by RT-PCR using total RNA extracted from the animal caps. AC, control
animal cap; WE, sibling whole embryos. D and E, knockdown of Hspa5 suppresses lhx1 expression. Either 30 ng of Hspa5MO1 (MO1) (D) or Hspa5MO2 (MO2) (E)
was co-injected with 100 pg of LacZ mRNA into one ventral animal blastomere of four-cell stage embryos. Injected embryos were collected at stage 15. The
injected side was identified by red X-Gal staining, and the expression of lhx1 was examined by whole mount in situ hybridization. Image published in: Shi W et al. (2015) Copyright © 2015. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License.
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