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FIGURE 7. Depletion of HSPA5 induces down-regulation of RAR at protein level. A, real time PCR indicates the expression of genes involved in RA signaling and atRA metabolism in control (blue column) or in HSPA5-depleted HEK293T cells (red column). B–F3, knockdown of HSPA5 reduced RAR protein in SH-SY5Y as revealed by confocal imaging. Either HSPA5 siRNA or control siRNA was transfected into SH-SY5Y cells. The knockdown efficiency is confirmed by Western blotting (B). C–F3, confocal imaging illustrates the subcellular expression of endogenous HSPA5 (green; C1–F1) and RAR (red; C2–F2) in SH-SY5Y cells. The nucleus was stained with DAPI (blue; C–F), and the merged images are shown in C3–F3. A clear reduction of RAR was identified in HSPA5-depleted cells (D2 and F2) compared with that in control (con) siRNA-transfected cells (C2 and E2). C–D3, lower magnification; E–F3, high magnification. Scale bars represent the length as indicated. Image published in: Shi W et al. (2015) Copyright © 2015. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Larger Image Printer Friendly View

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