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FIGURE 8. Lhx1 expression partially rescues pronephros inhibition induced by Hspa5MO. A–H , expression of pronephric markers including lhx1, pax2, nephrin, and smp30 in embryos injected with either Hspa5MO1 (MO1) (30 ng) or a mixture of Hspa5MO1 (30 ng) and lhx1 (100 pg) were examined by whole mount in situ hybridization. Single ventral-vegetal blastomeres of eight-cell stage embryos were targeted, and LacZ mRNA was used as a lineage tracer. A, C, E, and G, Hspa5MO-injected side, A , C , E , and G , non-injected (Non-inj) side of the same embryo. B, D, F, and H, Hspa5MO1- and lhx1-injected side. B , D , F , and H , non-injected side. I, quantification of the rescue experiments of different pronephros markers. The phenotype assessments were performed blinded to the treatment. The numbers on the tops of the columns indicate the total number of injected embryos. Image published in: Shi W et al. (2015) Copyright © 2015. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Gene Synonyms Species Stage(s) Tissue lhx1.L lim-1, Lim1, lim1, Xlim-1, Xlim1 X. laevis Throughout NF stage 32 pronephric kidney pax2.L LOC108697493, pax-2, pax2-a, pax2-b, XPax-2, XPax2 X. laevis Throughout NF stage 32 pronephric kidney nphs1.S nephrin X. laevis Throughout NF stage 32 pronephric mesenchyme rgn.L regucalcin, smp30, xsmp-30, xsmp30 X. laevis Throughout NF stage 32 pronephric kidney Image source: Published Larger Image Printer Friendly View

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