Xenbase Image ID: 139021

Fig. 2. Tracing myeloid cell migration. (A) Representative still images of dual channel confocal projection time-lapse microscopy, following one mpeg+ macrophage-like cell (white arrowhead). A target cell (a necrotic/apoptotic GFP+ granulocyte), which is engulfed by a macrophage, is denoted and the time of the sequence frames is labeled at the top right corner. See supplementary video 7, associated with this panel. (B) Single cell tracking of the mpeg+ cell from panel A was made with the Mtrack2 plug-in for ImageJ. Time points were acquired each 2 min and the Cartesian coordinates of the track were plotted as red dots. Time point counterparts with the panel A are indicated. (C) Point speed of migration (red line) was calculated for each time point and the average speed through the time lapse is denoted as a dashed line. (D) Representative still images of dual channel confocal projection time lapse pointing at a lurp+ granulocyte-like cell (white arrowhead). An excisional wound was made at the top right corner and the time labels denote minutes after wounding. See supplementary video 8, associated with this panel. (E) Single cell track data was extracted from the time lapse showed in panel D and time point labels are added as guidance. (F) Point speed of migration (red line) was calculated for each time point and the average speed through the time lapse is denoted as a dashed line. Image published in: Paredes R et al. (2015) © 2015 The Authors. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution license Larger Image Printer Friendly View

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