Xenbase Image ID: 139023

Fig. 4. Wound healing after injury in Xenopus larvae. (A) Schematic representation of the forceps piercing and biopsy punch wound assays used in transgenic lurp1:GFP X. laevis larvae. Myeloid cells are labeled in green and fin capillary network is labeled in red. (B)–(D) Single channel confocal projection and best in focus transmitted light image merge of a forceps pierced wound (dark area at the center) at 5 min, 7 days and 14 days after wounding, respectively. (E) and (F) Single channel confocal projection and best in focus transmitted light image merge of a biopsy punch wound (excisional wound at the center) at 2 h after wounding. GFP+ cells are labeled myeloid cells (E) and the capillary network is highlighted with a red mask. (G) and (H) Single channel confocal projection and best in focus transmitted light image merge of the healed wound 7 days after injury. GFP+ cells are labeled myeloid cells (G). The capillary network is highlighted with a red mask (old blood vessels) and an orange mask (new blood vessels). (I) Schematic representation of a biopsy punch wounding assays in transgenic mpeg1:GFP X. laevis larvae. (J) and (K) Single channel confocal projection and best in focus transmitted light image merge of a healed wound 8 days after injury. GFP+ cells are labeled macrophage-like cells. The excisional wound was originally at the center of the field. Image published in: Paredes R et al. (2015) © 2015 The Authors. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution license Larger Image Printer Friendly View

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