Xenbase Image ID: 139028

Fig. 9. Granulocyte response to bacterially infected wounds. (A) Schematic representation of the wounding assay plus bacteria infection in lurp1:GFP transgenic Xenopus larva. The puncher was loaded with live E. coli (red tip) before generating the excisional wounds. (B) Single-channel confocal projection merged with the best in focus transmitted light image of the infected wound. Granulocyte-like cells are labeled in green and the excised tissue area is highlighted in red. See supplementary video 14, associated with this panel. (C) Average speed was calculated from the tracks of lurp+ cells from at least 3 different experiments for each condition (1 h after wounding). Mann–Whitney U-test, *p<0.05. (E) The tracks obtained from the lurp+ cells at 1 h and 4 h after wounding were plotted forcing a common origin in a quadrant mesh. (F) and (G) The magnitude and the angles of the displacement vectors obtained from the lurp+ cell tracks 1 h after biopsy punch-wounding assay were plotted in rose charts (MatLab). The location of the wound site is denoted by a pink overshadow. Rayleigh test (CircStat for MatLab, The MatWorks) p-value is also indicated. (H) and (I) The magnitude and the angles of the displacement vectors obtained from the lurp+ cell tracks 4 h after biopsy punch-wounding assay were plotted in rose charts (MatLab). The location of the wound site is denoted by a pink overshadow. Rayleigh test (CircStat for MatLab, The MatWorks) p-value is also indicated. Image published in: Paredes R et al. (2015) © 2015 The Authors. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution license Larger Image Printer Friendly View

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