XB-IMG-139069
Xenbase Image ID: 139069
|
Fig. 1. Sulf1 inhibits the ability of Wnt8a to activate canonical Wnt signalling. (A–M) Xenopus laevis embryos were microinjected with mRNA encoding Wnt8a
(5 pg) and/or Sulf1 (1 ng) into a single ventral blastomere at the four-cell stage. (A–F) Whole embryo phenotypes of (A) uninjected control embryo, and embryos
injected with (B) Sulf1, (C) Wnt8a or (D–F) Sulf1 and Wnt8a. White arrowheads in C mark a fully duplicated axis; red arrowheads in F mark duplicated trunk
and cement gland. (G) The data shown in A–F is quantified in G. **P,0.01 (Chi squared test). (H–M) In situ hybridisation shows the expression of chordin at NF
stage 10.5. (H) Uninjected control embryos; (I–K) embryos injected with (I) Sulf1, (J) Wnt8a and (K) Sulf1 and Wnt8a. (L) Wnt8a induces an ectopic domain of
chordin expression. (M) Sulf1 inhibits the ability of Wnt8a to induce ectopic chordin resulting in the formation of a partial secondary organiser domain. The red
arrowheads in L and M mark the edges of the ectopic chordin domains. (N) The data shown in H–M is quantified in N, **P,0.01 (Chi squared test). (O–U) In situ
hybridisation shows the expression of Xnr3 at NF stage 10. (O) Uninjected control embryos; (P–T) embryos injected with (P) Sulf1, (Q) Wnt8a and (R) Sulf1 and
Wnt8a. Enlarged images of embryos shown in Q and R are shown in S and T. The data shown in O–T is quantified in (U). N, number of embryos. Image published in: Fellgett SW et al. (2015) © 2015. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution license
Image source: Published Larger Image Printer Friendly View |