XB-IMG-139072
Xenbase Image ID: 139072
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Fig. 4. Sulf1 enhances the ability of Wnt11b to activate both canonical and non-canonical Wnt signalling. (A–F) Xenopus laevis embryos were
microinjected with mRNA encoding Wnt11b (600 pg) and/or Sulf1 (1 ng) into a single ventral blastomere at the four-cell stage. In situ hybridisation for the gene
chordin was performed at NF stage 10.5. (A) Uninjected control embryos; (B–D) embryos injected with (B) Sulf1, (C) Wnt3a or (D) Sulf1 and Wnt11b. The areas
indicated in the white boxes in in C and D are enlarged in E and F, respectively. (G) The data in A–F is quantified in G. **P,0.01 (Chi squared test). (H–L)
Embryos were microinjected bilaterally in the animal hemisphere with mRNA encoding Wnt11b (50 pg) and Sulf1 (500 pg). Embryos were cultured until NF
stage 8, animal explants were taken and cultured until NF stage 10.5 in either the presence or absence of activin. (H,I) Control animal explants culture in either
the absence (H) or presence (I) of activin. (J–L) Animal explants injected with (J) Wnt11b, (K) Sulf1 or (L) Wnt11b and Sulf1. (M) The classification system used
to score animal cap explants shown in N. (N) The data shown in H–L is quantified in N. N, number of embryos. Image published in: Fellgett SW et al. (2015) © 2015. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution license
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