XB-IMG-139074
Xenbase Image ID: 139074
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Fig. 6. Sulf1 enhances the accumulation
of Wnt11b–GFP on the cell membrane.
(A–P) Xenopus laevis embryos were
microinjected bilaterally with mRNA
encoding mRFP (500 pg) into the animal
hemisphere at the two-cell stage. In
addition, embryos were injected with mRNA
encoding LacZ (4 ng), Sulf1 (4 ng), Wnt8a–
GFP (500 pg), Wnt11b–GFP (1 ng) or a
mixture of the four. (A–D) Control animal
explants overexpressing LacZ and Wnt8a–
GFP. (E–H) Animal explants
overexpressing Sulf1 and Wnt8a–GFP.
(I–L) Control animal explants
overexpressing LacZ and Wnt11b–GFP.
(M–P) Animal explants overexpressing
Sulf1 and Wnt11b–GFP. The white boxes in
C, G, K and O mark the areas that are
enlarged in D, H, L and P, respectively.
mRFP is shown in magenta, Wnt8a– or
Wnt11b–GFP is shown in green. Scale
bars: 20 mm. (Q,R) Graphs quantifying the
relative levels of (Q) Wnt8a–GFP and
(R) Wnt11b–GFP on the cell membrane.
Data was quantified using a programme
written in Matlab, results are mean6s.e.m.
**P,0.01; NS, not significant (Mann–
Whitney U test). N, number of embryos. Image published in: Fellgett SW et al. (2015) © 2015. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution license Larger Image Printer Friendly View |