Xenbase Image ID: 139078

Figure 2 [A-L] Xenopus laevis embryos were microinjected bilaterally with mRNA encoding mRFP (500pg) and Dvl-GFP (500pg) into the animal hemisphere at the two cell stage. In addition embryos were injected with increasing amounts of Wnt11b mRNA. [A-D] Control animal explants over expressing mRFP and Dvl-GFP. Animal explants injected with [E-H] Wnt11b (400pg) or [I-L] Wnt11b (800pg) mRNA. The white boxes in [C], [G] and [K] mark the areas used to create panels [D], [H] and [L] respectively. [M] The data shown in [A-L] is quantified in [M]. Quantification was done using a program written in MatLab, Mann-Whitney U (**P<0.01), error bars represent s.e.m, N=number of embryos. mRFP (magenta), Dvl-GFP (green), scale bars represent 20um. [N] A diagram of the ATF2 reporter used for this assay is shown above a graph depicting the results obtained when 100pg of ATF reporter plasmid DNA and 1pg of Renilla was injected into the marginal zone of all four cells of four cell stage Xenopus laevis embryos. In addition embryos were injected with mRNA encoding Sulf1 (4ng) or Wnt11b (200pg) with a control mRNA or with Wnt11b (200pg) together with Sulf1. Embryos were lysed and analysed in a luminometer at stage 10 to assay luciferase activity. The graph illustrates the effects of Sulf1 and Wnt11b on the activation of the ATF reporter. Image published in: Fellgett SW et al. (2015) © 2015. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution license Larger Image Printer Friendly View

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