Xenbase Image ID: 139082

Fig. 2. Neural induction in vivo by combined FGF signalling and BMP inhibition. (A-E) Sixteen-cell embryos were injected in one ventral-most animal blastomere with lacZ and 0.16 pg eFGF (A); lacZ, 0.16 pg eFGF and 1 ng Smad6 (B,C); lacZ, 0.16 pg eFGF and 200 pg tBR (D); or lacZ, 1 ng Smad6 and 1 pg v-ras RNAs (E). Low eFGF or activated ras signalling induces neural tissue, without Xbra- positive mesoderm, when combined with BMP inhibition in late gastrula stage 13 embryos. NCAM immunostaining at tailbud stage confirms the stable neural character of the induced tissue (arrow in C). (A,B,E) Ventral view, anterior towards the top; (C) lateral view, anterior towards the right; (D) lateral view, anterior towards the top. Scale bars: 500 μm. Image published in: Delaune E et al. (2005) Copyright © 2005. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Larger Image Printer Friendly View

Return to previous page